منابع مشابه
Chitin and yeast budding. Properties of chitin synthetase from Saccharomyces carlsbergensis.
A particulate preparation from a spheroplast lysate of Saccharomyces cartsbergensis was found to catalyze the transfer of acetylglucosamine from UDP-acetylglucosamine to’an endogenous acceptor. Uridine diphosphate is liberated in stoichiometric amounts. A divalent cation, MgZ+, Mn2+, or Co2+, is required for enzymatic activity. Acetylglucosamine stimulates the activity about 5-fold, with a K, o...
متن کاملSeptins, under Cla4p regulation, and the chitin ring are required for neck integrity in budding yeast.
CLA4, encoding a protein kinase of the PAK type, and CDC11, encoding a septin, were isolated in a screen for synthetic lethality with CHS3, which encodes the chitin synthase III catalytic moiety. Although Ste20p shares some essential function with Cla4p, it did not show synthetic lethality with Chs3p. cla4 and cdc11 mutants exhibited similar morphological and septin localization defects, includ...
متن کاملChitin synthase III activity, but not the chitin ring, is required for remedial septa formation in budding yeast.
Chitin is a minor but essential component of the Saccharomyces cerevisiae cell wall. In wild-type, chitin synthase II is required for the formation of primary septa and chitin synthase III (CSIII) is not essential. However, in chs2 mutants CSIII becomes essential for the formation of aberrant septa. We examined which of two CSIII functions, the formation of a chitin ring at bud emergence or of ...
متن کاملMitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast
How cell cycle machinery regulates extracellular matrix (ECM) remodeling during cytokinesis remains poorly understood. In the budding yeast Saccharomyces cerevisiae, the primary septum (PS), a functional equivalent of animal ECM, is synthesized during cytokinesis by the chitin synthase Chs2. Here, we report that Dbf2, a conserved mitotic exit kinase, localizes to the division site after Chs2 an...
متن کاملRNAi in budding yeast.
RNA interference (RNAi), a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast Saccharomyces cerevisiae. Here, we show that RNAi is present in other budding yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate small interfering ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62543-8